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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
27/05/2009 |
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Data da última atualização: |
05/06/2018 |
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Autoria: |
CANGAHUALA-INOCENTE, G. C.; STEINER, N.; MALDONADO, S. B.; GUERRA, M. P. |
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Afiliação: |
Gabriela Claudia Cangahuala?Inocente, Universidade Federal de Santa Catarina, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós?graduação em Recursos Genéticos Vegetais; Neusa Steiner, Universidade Federal de Santa Catarina, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós?graduação em Recursos Genéticos Vegetais; Sara Beatriz Maldonado, Universidad de Buenos Aires, Departamento de Biodiversidad y Biología Experimental; Miguel Pedro Guerra, Universidade Federal de Santa Catarina, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós?graduação em Recursos Genéticos Vegetais. |
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Título: |
Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 3, p. 217-224, mar. 2009. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Padrões de acúmulo de proteínas e carboidratos durante a embriogênese somática de Acca sellowiana. |
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Conteúdo: |
The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid?Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana. |
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Palavras-Chave: |
açúcares totais; goiabeira serrana; pineapple guava; total sugars. |
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Thesagro: |
Amido; Metabolismo. |
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Thesaurus Nal: |
metabolism; starch. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/38043/1/44n03a01.pdf
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Marc: |
LEADER 02241naa a2200265 a 4500
001 1125724
005 2018-06-05
008 2009 bl uuuu u00u1 u #d
100 1 $aCANGAHUALA-INOCENTE, G. C.
245 $aPatterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana.
260 $c2009
500 $aTítulo em português: Padrões de acúmulo de proteínas e carboidratos durante a embriogênese somática de Acca sellowiana.
520 $aThe aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid?Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana.
650 $ametabolism
650 $astarch
650 $aAmido
650 $aMetabolismo
653 $aaçúcares totais
653 $agoiabeira serrana
653 $apineapple guava
653 $atotal sugars
700 1 $aSTEINER, N.
700 1 $aMALDONADO, S. B.
700 1 $aGUERRA, M. P.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 44, n. 3, p. 217-224, mar. 2009.
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Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
10/07/2014 |
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Data da última atualização: |
01/06/2017 |
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Autoria: |
BORGES, B. O.; CURI, R. A.; BALDI, F.; FEITOSA, F. L. B.; ANDRADE, W. B. F. de; ALBUQUERQUE, L. G. de; OLIVEIRA, H. N. de; CHARDULO, L. A. L. |
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Título: |
Polymorphisms in candidate genes and their association with carcass traits and meat quality in Nellore cattle. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 5, p. 364-371, maio. 2014. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Polimorfismos em genes-candidatos e suas associações com características de carcaça e qualidade da carne em bovinos Nelore. |
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Conteúdo: |
The objective of this work was to estimate the allele polymorphism frequencies of genes in Nellore cattle and associate them with meat quality and carcass traits. Six hundred males were genotyped for the following polymorphisms: DGAT1 (VNTR with 18 nucleotides at the promoter region); ANK1, a new polymorphism, identified and mapped here at the gene regulatory region NW_001494427.3; TCAP (AY428575.1:g.346G>A); and MYOG (NW_001501985:g.511G>C). In the association study, phenotype data of hot carcass weight, ribeye area, backfat thickness, percentage of intramuscular fat, shear force, myofibrillar fragmentation index, meat color (L*, a*, b*), and cooking losses were used. Allele B from the ANK1 gene was associated with greater redness (a*). Alleles 5R, 6R, and 7R from the DGAT1 VNTR gene were associated with increased intramuscular fat, reduced cooking losses and increased ribeye area, respectively. The single nucleotide polymorphism (SNP) of the TCAP gene was not polymorphic, and MYOG alleles were not associated with any of the evaluated characteristics. These results indicate that ANK1 and DGAT1 genes can be used in the selection of Nellore cattle for carcass and meat quality. |
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Palavras-Chave: |
Anquirina; Assisted selection; Miogenina; Seleção assistida; Titina. |
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Thesagro: |
Bos Indicus; Gado de corte. |
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Thesaurus NAL: |
Ankyrins; Beef cattle; myogenin. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/104887/1/Polymorphisms-in-cadidate-genes.pdf
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Marc: |
LEADER 02300naa a2200337 a 4500
001 1990164
005 2017-06-01
008 2014 bl uuuu u00u1 u #d
100 1 $aBORGES, B. O.
245 $aPolymorphisms in candidate genes and their association with carcass traits and meat quality in Nellore cattle.
260 $c2014
500 $aTítulo em português: Polimorfismos em genes-candidatos e suas associações com características de carcaça e qualidade da carne em bovinos Nelore.
520 $aThe objective of this work was to estimate the allele polymorphism frequencies of genes in Nellore cattle and associate them with meat quality and carcass traits. Six hundred males were genotyped for the following polymorphisms: DGAT1 (VNTR with 18 nucleotides at the promoter region); ANK1, a new polymorphism, identified and mapped here at the gene regulatory region NW_001494427.3; TCAP (AY428575.1:g.346G>A); and MYOG (NW_001501985:g.511G>C). In the association study, phenotype data of hot carcass weight, ribeye area, backfat thickness, percentage of intramuscular fat, shear force, myofibrillar fragmentation index, meat color (L*, a*, b*), and cooking losses were used. Allele B from the ANK1 gene was associated with greater redness (a*). Alleles 5R, 6R, and 7R from the DGAT1 VNTR gene were associated with increased intramuscular fat, reduced cooking losses and increased ribeye area, respectively. The single nucleotide polymorphism (SNP) of the TCAP gene was not polymorphic, and MYOG alleles were not associated with any of the evaluated characteristics. These results indicate that ANK1 and DGAT1 genes can be used in the selection of Nellore cattle for carcass and meat quality.
650 $aAnkyrins
650 $aBeef cattle
650 $amyogenin
650 $aBos Indicus
650 $aGado de corte
653 $aAnquirina
653 $aAssisted selection
653 $aMiogenina
653 $aSeleção assistida
653 $aTitina
700 1 $aCURI, R. A.
700 1 $aBALDI, F.
700 1 $aFEITOSA, F. L. B.
700 1 $aANDRADE, W. B. F. de
700 1 $aALBUQUERQUE, L. G. de
700 1 $aOLIVEIRA, H. N. de
700 1 $aCHARDULO, L. A. L.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 49, n. 5, p. 364-371, maio. 2014.
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